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KMID : 0043320150380081552
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Archives of Pharmacal Research
2015 Volume.38 No. 8 p.1552 ~ p.1560
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EPA attenuates ultraviolet radiation-induced downregulation of aquaporin-3 in human keratinocytes
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Jeon Byoung-Kook
Kang Moon-Kyung
Lee Ghang-Tai
Lee Kun-Kuk
Lee Ho-Sub
Woo Won-Hong
Mun Yeun-Ja
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Abstract
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Eicosapentaenoic acid (EPA) is an omega-3 polyunsaturated fatty acid (ω-3 PUFA) that protects against photodamage and photocarcinogenesis in mammals. Aquaporin-3 (AQP3) is a water/glycerol transport protein that is found in basal layer keratinocytes. In this study, we have investigated the protective effect of EPA against ultraviolet B (UVB)-induced AQP3 downregulation in human keratinocytes. EPA treatment was found to increase AQP3 gene and protein expression in human epidermal keratinocytes (HaCaT). Using a specific inhibitor, we observed that the effect of EPA on AQP3 expression was mediated by extracellular signal-regulated kinase (ERK) activation. UVB radiation induced AQP3 downregulation in HaCaT cells, and it was found that EPA treatment attenuated UVB-induced AQP3 reduction and the associated cell death. UVB-induced downregulation of AQP3 was blocked by EPA and p38 inhibitor SB203580. Collectively, the present results show that EPA increased AQP3 expression and that this led to a reduction UVB-induced photodamage.
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KEYWORD
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Aquaporin-3, EPA, UVB, ERK activation, Human keratinocyte
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